Skip to main content

Week 8 - 8/19

By
On Monday, I got to interview Jason. He had a lot of great advice for me for my future. We also discussed how I could continue to work on this throughout the school year. I also used the last and final plate on my LNCAP cells on the Cellvo plates. I will have to do one more split after this to get my final RNA and Protein data and I am super hopeful that I can do it at the end of the week. That way, I can have done a full experiment on my own.

On Tuesday, I made my first plasmids. This was really cool. I injected DNA into E. Coli cells so hopefully, they will reproduce it. I also ran RNA extraction on my sample from yesterday and RT.

On Wednesday, I looked at the plasmid we set to cure overnight. It was super cool. I also worked on getting organized and preparing to end my time in the lab. I am really hoping that I can get everything done but I don't believe I will have quite enough time. Hopefully, I can come in one Saturday so that I can complete my research project start to end.

On Thursday, I discovered that most of my plasmids did not work from Tuesday. I redid them today. I also took a look at my LNCAP cells and realized that they were not ready to split yet.

On Friday, we went out for lunch to celebrate my last day. I am sad that my time in the lab has come to a close.

Comments

Post a Comment

Popular posts from this blog

All the pictures

By
Consortium Poster Dr. Somarelli and I with my poster creating a gel my cells!!! qPCR test Cells in Cellvo plate Splitting cells in Tissue Culture cells in the regular plate cells colonizing Western Blot in the process of running Loading a Western Blot
Post a Comment
Read more

About Me

By
 Hello, My name is Elyse Schechter. I go to Apex High School (roll cougs) where I am a part of the Academy of Information Technology (AOIT) program. In this program, I learned very important soft skills such as interviewing, communicating, and presenting along with hard skills such as computer coding. In this program, I have become a much more confident public speaker and also became Microsoft Word and PowerPoint 2016 certified. I have been exposed to 4 different coding languages (visual basic, C#, SAS, and java) and given the opportunity to participate in an internship at a place of my choice. I interned at the Duke Cancer Institute, which is why this blog was started. Part of my assignments for this internship was to write updates for every 5-10 hours of work and write about what I enjoyed about the workplace. 
Post a Comment
Read more

Reverse Transcription

By
As I was writing previous posts I realized I did not make one about RT or Reverse Transcription. To explain, I must first describe transcription. Transcription is the process of how DNA is turned into RNA. Therefore RT is the process of RNA being turned back into DNA, or more specifically cDNA (coding DNA).
Post a Comment
Read more