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Week 8 - 8/19

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On Monday, I got to interview Jason. He had a lot of great advice for me for my future. We also discussed how I could continue to work on this throughout the school year. I also used the last and final plate on my LNCAP cells on the Cellvo plates. I will have to do one more split after this to get my final RNA and Protein data and I am super hopeful that I can do it at the end of the week. That way, I can have done a full experiment on my own.

On Tuesday, I made my first plasmids. This was really cool. I injected DNA into E. Coli cells so hopefully, they will reproduce it. I also ran RNA extraction on my sample from yesterday and RT.

On Wednesday, I looked at the plasmid we set to cure overnight. It was super cool. I also worked on getting organized and preparing to end my time in the lab. I am really hoping that I can get everything done but I don't believe I will have quite enough time. Hopefully, I can come in one Saturday so that I can complete my research project start to end.

On Thursday, I discovered that most of my plasmids did not work from Tuesday. I redid them today. I also took a look at my LNCAP cells and realized that they were not ready to split yet.

On Friday, we went out for lunch to celebrate my last day. I am sad that my time in the lab has come to a close.

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