On Monday, I did my presentation for the U54 meeting. The presentation went well! Afterward, I split my cells again for the third split. I was able to complete RNA and Protein extraction after the split. I also was able to determine the amount of RNA in my samples so I can run an RT tomorrow morning.
On Tuesday, I finished the RT from my RNA samples from Monday. I also ran more Western Blots for Sarah. I've noticed that I am getting pretty good at doing the basics of Western Blots/RNA extraction/Protein extraction/Splitting Cells. I feel like this will be really useful for when I go to college and I will already know how to do the basics.
On Wednesday, I ran RT on one of Beas samples that I had extracted RNA from last week. I learned the importance of labeling samples. Each sample was in triplicate and I did not originally distinguish which of the three was which. So the data I had on how much RNA in each sample meant nothing. I am glad I learned this lesson here on something that was an easy and quick fix. All I needed to do is relabel my samples and run a different RNA quantification.
On Tuesday, I finished the RT from my RNA samples from Monday. I also ran more Western Blots for Sarah. I've noticed that I am getting pretty good at doing the basics of Western Blots/RNA extraction/Protein extraction/Splitting Cells. I feel like this will be really useful for when I go to college and I will already know how to do the basics.
On Wednesday, I ran RT on one of Beas samples that I had extracted RNA from last week. I learned the importance of labeling samples. Each sample was in triplicate and I did not originally distinguish which of the three was which. So the data I had on how much RNA in each sample meant nothing. I am glad I learned this lesson here on something that was an easy and quick fix. All I needed to do is relabel my samples and run a different RNA quantification.
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