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My Projects

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I have three projects I will start focusing on. The first one is to rerun a few experiments Bea did to verify her results. This will involve three cell lines LNCap, CS2, LN95, all of which are prostate cancers. There will then be two types of each cell, the control and a cell group that is enzalutamide resistant. Enzalutamide is a drug that is often used first when treating prostate cancer. Therefore the cells that are resistant to it are a good demonstration of cancer cells that are drug resistant and we can see how it might react to other drugs similar to in patients. I will run dose curves on the drug we are testing and run western blots and qPCRs. The second project will be experimenting with a new type of cell plate. These cell plates are supposed to mimic bones and the idea is that it will help cells grow better since it better mimics the body. It is also good for prostate cancer because the bone is where it tends to metastasize to. I will eventually run 5 splits on a normal plate and a new plate. With each split 1/5 will be replated, 1/5 will be collected for RNA extraction, and 3/5 will be collected for protein extraction. The point of this is to see if there are any differences in the cells gene expression between plates. My third project is to test a new drug on cells. I will run IC50 western blots and qPCRs in order to look at the new drugs effect on the cells.

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