Skip to main content

qPCR

By
qPCR is a test that shows gene expression over time. In Dukes lab, we run this test on a 384 well plate. Each well will end up with 2ul of cDNA or coding DNA and 8ul of master mix. This is obtained from a process called RT or reverse transcription. In qPCR, you must first dilute your cDNA with water. Then a master mix must be created for each thing you want to look at in qPCR. This master mix is made of water, a forward and reverse primer and a reagent. There is a guide to a mix solution for one individual sample so it must be multiplied by however many samples you are testing plus one or two for pipet error. For example, I was running 12 samples so I multiplied by 15 in order to be sure I had enough of the mixture. Every sample is mixed in their individual well, a film is placed over the plate and it is placed in the centrifuge. Finally it is put in the qPCR machine which analyzes the gene expression.

Comments

Post a Comment

Popular posts from this blog

All the pictures

By
Consortium Poster Dr. Somarelli and I with my poster creating a gel my cells!!! qPCR test Cells in Cellvo plate Splitting cells in Tissue Culture cells in the regular plate cells colonizing Western Blot in the process of running Loading a Western Blot
Post a Comment
Read more

Review Paper

By
One of my other projects is to help write a review paper on marine derived anti-cancer compounds. I learned a review paper is essentially a collection of others work put together with the authors own ideas and thoughts on how it could be used. We are writing our paper around the set up of the "Hallmarks of Cancer" paper. This is a very well known paper that discusses the characteristics that all cancers usually have. Not only does it list the characteristics of cancer, it describes how each thing occurs to the best of the knowledge they have. My part in this was to write the introduction and conclusion. I am on a team with 3 other college students who are doing research with Dr. Somarelli. They are all very helpful and answer my questions about not only the review paper or related subjects, but about college in general. This experience has shown me what it is like to work with other college students for undergraduate research. I have learned a lot and it has helped me signif...
Post a Comment
Read more

About Me

By
 Hello, My name is Elyse Schechter. I go to Apex High School (roll cougs) where I am a part of the Academy of Information Technology (AOIT) program. In this program, I learned very important soft skills such as interviewing, communicating, and presenting along with hard skills such as computer coding. In this program, I have become a much more confident public speaker and also became Microsoft Word and PowerPoint 2016 certified. I have been exposed to 4 different coding languages (visual basic, C#, SAS, and java) and given the opportunity to participate in an internship at a place of my choice. I interned at the Duke Cancer Institute, which is why this blog was started. Part of my assignments for this internship was to write updates for every 5-10 hours of work and write about what I enjoyed about the workplace. 
Post a Comment
Read more